CGSC Strain#: 5167
Strain Designation: D31m4      Source of Strain: H.G. Boman
Sex: F- Chromosomal Markers: proA23, lac-28, tsx-81, trp-30, his-51, rpsL174(strR), rfa-229, rfa-230, ampCp-1
Strain Comments:

• rfa-230 was formerly called lps-30.
• ampCp-1-- is a promoter mutation; AmpC⁺ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
• ampCp-1 was formerly called ampA1
• rfa-229 was formerly called lps^-
• strR = streptomycin resist.

References:

• Monner, DA, S Jonsson, HG Boman 1971. Ampicillin-resistant mutants of Escherichia coli K-12 with lipopolysaccharide alterations affecting mating ability and susceptibility to sex-specific bacteriophages. J. Bacteriol. 107(2):420-32.
• Boman, HG, DA Monner 1975. Characterization of lipopolysaccharides from Escherichia coli K-12 mutants. J. Bacteriol. 121(2):455-64.
• Strain, SM, SW Fesik, IM Armitage 1983. Characterization of lipopolysaccharide from a heptoseless mutant of Escherichia coli by carbon 13 nuclear magnetic resonance. J. Biol. Chem. 258(5):2906-10.