CGSC Strain#: 7743
Strain Designation: A50      Source of Strain: Russel & Model
Sex: F- Chromosomal Markers: garB10, fhuA22, phoA4(Am), ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, zid-5::Tn5, rrnB-2, mcrB1, creC510
Strain Comments:

• Reference for zid-5 insertion in strain A34.
• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• = kanamycin resistant
• Am = amber(UAG) mutation
• Const = constitutive
• T2R = T2 Resistant

References:

• Russel, M., P. Model 1983. A bacterial gene, fip, required for filamentous bacteriophage F1 assembly. J.Bacteriol. 154:1064-1076