CGSC Strain#: 6642
Strain Designation: CLB7      Source of Strain: C.L. Bassett
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), lacY1, rna-19, λ^-, galU536, recB21, pnp-7, rpsL132(strR), malT1(λ^R), xyl-7, mtlA2, thiE1
Strain Comments:

• This strain is Thr⁺. Either it does not carry thr-1 or the marker is very leaky in this background.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• rna-19-- : Additional references to strain A19 and its derivatives: \\ Gesteland 1966 J.Mol.Biol.16:67 \\ Reiner 1969 J.Bacteriol. 97:1431,1437,1522
• rna-19-- : Also References \\ Weatherford et al. 1972 J.Biol.Chem.247:540 \\ Studier 1975 J.Bacteriol.124:307 \\ Apirion and Watson 1975 J.Bacteriol.124:317
• galU536-- : This strain was reported to be Gal^-, apparently due to a spontaneous galU mutation.
• rna-19 was formerly called rns-19
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• Am = amber(UAG) mutation
• LamR = Lambda-resistant
• strR = streptomycin resist.

References:

• Bassett, CL, JR Rawson 1983. In vitro coupled transcription-translation of linear DNA fragments in a lysate derived from a recB rna pnp strain of Escherichia coli. J. Bacteriol. 156(3):1359-62.