CGSC Strain#: 6599
Strain Designation: RC30      Source of Strain: J.R. Christensen
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: ΔlacZ58(M15), lacI22, λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• ΔlacZ58(M15)-- is a deletion of a small part (the alpha region) of the lacZ gene; allows `alpha complementation'.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• lacI22 was formerly called lacI3
• lacI22 was formerly called lac_i3^-
• ΔlacZ58(M15) was formerly called deletion M15
• ΔlacZ58(M15) was formerly called lacZ(M15)
• thiE1 was formerly called thi-1
• M15 = alpha-complemented allele

References:

• BECKWITH, JR 1964. A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI. J. Mol. Biol. 8:427-30.