CGSC Strain#: 6595
Strain Designation: pLC25-33/WL8      Source of Strain: J. Walsh
Sex: F- Episome/Plasmid: pLC25-33
   Plasmid Markers/Mutations: araC14, thr-1, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ^-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1, melA , melB , fumB
   Plasmid Comment: USE CGSC No. 6595 Hybridizes with Kohara clones 644-647.
Chromosomal Markers: recA56, srlD300::Tn10, relA1, spoT1, metB1, melA7
Strain Comments:

• Genes identified on plasmid pLC25-33: melA, melB, fumB
• recA56-- Amino acid substitution Arg 60 Cys
• srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• srlD300::Tn10 was formerly called srlC300::Tn10
• = tetracycline resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
• Tsuchiya, T, K Ottina, Y Moriyama, MJ Newman, TH Wilson 1982. Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli. J. Biol. Chem. 257(9):5125-8.
• Guest, J.R., J.S. Miles, R.E. Roberts, S.A. Woods 1985. The fumarase genes of Escherichia coli: location of the fumB gene and discovery of a new gene (fumC). J.Gen.Microbiol. 131:2971-2984