CGSC Strain#: 6594
Strain Designation: pLC17-38B/WL8      Source of Strain: J. Walsh
Sex: F- Episome/Plasmid: pLC28-38B
   Plasmid Markers/Mutations: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ^-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1
   Plasmid Comment: Isolated originally as mixed clone pLC28-38, carrying {\it rec} region (ca. 60 min.) as well as {\it melA-pheU} region at ca.93.9 min. {\it melAB} plasmid isolated in pLC28-38B/WL8
Chromosomal Markers: recA56, srlD300::Tn10, relA1, spoT1, metB1, melA7
Strain Comments:

• recA56-- Amino acid substitution Arg 60 Cys
• srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• srlD300::Tn10 was formerly called srlC300::Tn10
• = tetracycline resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References: