CGSC Strain#: 8035
Strain Designation: EDCM475      Source of Strain: Masters and Merlin
Sex: F- Chromosomal Markers: Δlac, λ^-, Δfnr-267, ΔyaeF-2, rph-1
Strain Comments:

• Δfnr-267-- was reported for MG1655 by pers.commun.from V.Stewart to B.Bachmann,1986, again to CGSC by V.S.2003. Some MG1655 subcultures and derivatives have it .See also Soupene et al. JBact.185:5611(2003).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)

References:

• Merlin, C., G. Gardiner, S. Durand, M. Masters 2002. The Escherichia coli metD locus encodes an ABC transporter which includes Abc (MetN), YaeE (MetI), and YaeC (MetQ). J.Bacteriol. 184:5513-5517
• Merlin, C., S. McAteer, M. Masters 2002. Tools for characterization of Escherichia coli genes of unknown function. J.Bacteriol. 184:4573-4581