CGSC Strain#: 6585
Strain Designation: FB2      Source of Strain: P. Carl
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: rnhA1, lacZ482(Am), λ^-, relA1, spoT1, rha-9, thiE1
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation

References:

• Carl, P. 1980. Isolation and mapping of a mutation in Escherichia coli with altered levels of ribonuclease H. J.Bacteriol. 144:28-35
• Arendes, J., P. Carl, A. Sugino 1982. A mutation in the rnh-locus of Escherichia coli affects the structural gene for RNase H. Examination of the mutant and wild type protein. J.Biol.Chem. 257:4719-4722