CGSC Strain#: 5414
Strain Designation: CR      Source of Strain: D. Denhardt
Sex: F- Chromosomal Markers: glnV0(AS), λ^-, thyA0, rpsL154(strR), φX^s, ThyR-(deoB,deoC)
Strain Comments:

• from a cross between Edgar's ``CR34 Hfr'' strain and Bertani's E. coli C strain C416.
• We have not yet been able to trace the derivation of this strain completely. It can be used to transfer φX-sensitivity by transduction with xyl⁺, although it does not grow φX very well.
• thyA0-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• φX^s-- : φX sensitivity is derived from E. coli C
• glnV0(AS) was formerly called supE-
• AS = amber(UAG) suppressor
• deoB,deoC = Old name thyR = deoB,deoC
• strR = streptomycin resist.

References:

• Denhardt, DT, RL Sinsheimer 1965. The process of infection with bacteriophage phi-X174. IV. Replication of the viral DNA in a synchronized infection. J. Mol. Biol. 12(3):647-62.
• Denhardt, DT, DH Dressler, A Hathaway 1967. THE ABORTIVE REPLICATION OF PhiX174 DNA IN A RECOMBINATION-DEFICIENT MUTANT OF Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 57(3):813-820.
• McFadden, G, DT Denhardt 1974. Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein. J. Virol. 14(5):1070-5.