CGSC Strain#: 5734
Strain Designation: RFS740      Source of Strain: R. Schleif
Sex: F' Episome/Plasmid: F101
   Plasmid Markers/Mutations:
Chromosomal Markers: Δ(araBAD-leu)498, Δ(codB-lacI)3, relA1, spoT1, thiE1
Strain Comments:

• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• thiE1 was formerly called thi-1

References:

• Schleif, R 1972. Fine-structure deletion map of the Escherichia coli L-arabinose operon. Proc. Natl. Acad. Sci. U.S.A. 69(11):3479-84.