CGSC Strain#: 4325
Strain Designation: KL176      Source of Strain: M.C. Cappello
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, e14-, gyrA12(NalR), recA1, relA1, recB21, thiE1, deoB13
Strain Comments:

• This strain does not mate with very high efficiency. It is necessary to plate out more cells than usual.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thiE1 was formerly called thi-1
• NalR = Nalidix.acidResist.

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