CGSC Strain#: 4415
Strain Designation: B3      Source of Strain: S. Brenner
Other Designations: EMG34
Other Designations: E. coli B Thy^-
Other Designations: B₃
Sex: F- Chromosomal Markers: thyA14
Strain Comments:

• Not a K-12 strain. This is a Thy^- derivative of E. coliB wild type.
• thyA14-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.

References:

• Clowes, R., W. Hayes 1968. Experiments in microbial genetics