CGSC Strain#: 4400
Strain Designation: 58-161, F-,SR      Source of Strain: W. Hayes
Other Designations: EMG1
Other Designations: RC502
Other Designations: F-Row
Other Designations: K12-Row
Other Designations: Row
Other Designations: CL142
Sex: F- Chromosomal Markers: relA1, rpsL100(strR), spoT1, metB1, creC510
Strain Comments:

• Called F-Row by Fredericq because he got it from Rowley.
• Same as the strain CL142, of Ozeki et al. (1959) and Lewis & Stocker (1965)
• This strain is often used as a colicin sensitive indicator strain
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• strR = streptomycin resist.

References:

• Hayes, W. 1953. Observations on a transmissible agent determining sexual differentiation in Bacterium coli. J.Gen.Microbiol. 8:72-88
• Clowes, R., W. Hayes 1968. Experiments in microbial genetics