CGSC Strain#: 8074
Strain Designation: MGΔLy      Source of Strain: M. Masters
Other Designations: MGdelLy
Sex: F- Episome/Plasmid: pBAD-L
   Plasmid Markers/Mutations: bla(ApR), ispH(lytB), ispH
Chromosomal Markers: ΔispH3::kan, λ^-, rph-1
Strain Comments:

• ispH is an essential gene and the presence of the gene on a plasmid is required for this strain to be viable. The strain may also be Fnr-minus.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant
• ApR = ampicillin resistant

References:

• McAteer, S., A. Coulson, N.F. McLennan, M. Masters 2001. The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis. J.Bacteriol. 183:7403-7407