CGSC Strain#: 4337
Strain Designation: KL333      Source of Strain: M.C. Cappello
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: leu-40, Δ(aroP-aceF)73, lacZ608(Am), λ^-
Strain Comments:

• leu-40-- is Norkin's leu-3.
• lacZ608(Am)-- is an amber mutation (Newton et al. 1965 J. Mol. Biol. 14:290), aka lacZ^-NG608 by Zinder, in segment 10 of the lacZ gene (Zipser et al. 1970).
• Am = amber(UAG) mutation

References:

• Newton, W.A., J.R. Beckwith, D. Zipser, S. Brenner 1965. Nonsense mutants and polarity in the lac operon of Escherichia coli. J.Mol.Biol. 14:290-296
• Norkin, L.C. 1970. Marker-specific effects in genetic recombination. J.Mol.Biol. 51:633-655
• Zipser, D, S Zabell, J Rothman, T Grodzicker, M Wenk 1970. Fine structure of the gradient of polarity in the z gene of the lac operon of Escherichia coli. J. Mol. Biol. 49(1):251-4.