CGSC Strain#: 8098
Strain Designation: CSH104      Source of Strain: J.H. Miller
Other Designations: CC104
Sex: F' Episome/Plasmid: F128-(CSH104)
   Plasmid Markers/Mutations: lacI373, lacZ574
Chromosomal Markers: ara-600, Δ(gpt-lac)5, λ^-, relA1, spoT1, thiE1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Cupples, C.G., J.H. Miller 1989. A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions. Proc.Natl.Acad.Sci.USA 86:5345-5349
• Miller, J.H. 1992. Short Course in Bact.Genet. CSH Press 1:431