CGSC Strain#: 4207
Strain Designation: KL161      Source of Strain: M.C. Cappello
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, e14-, relA1, thyA24, spoT1, thiE1, deoB13
Strain Comments:

• This strain has been tested for suppressors and none were found.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• thyA24-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References: