CGSC Strain#: 4206
Strain Designation: KL16-99      Source of Strain: M.C. Cappello
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, e14-, recA1, spoT1, thiE1, deoB13
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Low, K.B. 1968. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc.Natl.Acad.Sci.USA 60:160-167
• Sancar, A., C.S. Rupert 1978. Correction of the map location for the phr gene in Escherichia coli K12 Mutat. Res. 51(1):139-143.
• Sancar, A, CS Rupert 1978. Determination of plasmid molecular weights from ultraviolet sensitivities. Nature 272(5652):471-2.