CGSC Strain#: 8075
Strain Designation: EDCM636      Source of Strain: M. Masters
Sex: F- Chromosomal Markers: ΔcanA1::FLK2(lacZ,kan), λ^-, Δfnr-267, rph-1
Strain Comments:

• Δfnr-267-- was reported for MG1655 by pers.commun.from V.Stewart to B.Bachmann,1986, again to CGSC by V.S.2003. Some MG1655 subcultures and derivatives have it .See also Soupene et al. JBact.185:5611(2003).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant

References:

• Merlin, C., M. Masters, S. McAteer, A. Coulson 2003. Why is carbonic anhydrase essential to Escherichia coli?. J.Bacteriol. 185:6415-6424