CGSC Strain#: 2604
Strain Designation: HB27      Source of Strain: H. Boyer
Other Designations: AC2604
Sex: F+ Chromosomal Markers: leuB6(Am), Δ(gpt-proA)62, lacY1, galK2(Oc), rpsL20(strR), thiE1
Strain Comments:

• This is a hybrid E. coli K-12 x E. coliB strain with E. coliB restriction and modification.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Boyer, H 1966. Conjugation in Escherichia coli. J. Bacteriol. 91(5):1767-72.