CGSC Strain#: 4871
Strain Designation: DF40      Source of Strain: D. Fraenkel
Other Designations: L40
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, pgi-2, mcrB1, creC510
Strain Comments:

• We maintain this strain on a minimal medium containing 25 mM malate, 12.5 mM glycerol, and 0.1% casamino acids (Hillman and Fraenkel, 1975, J.Bacteriol.122:1175)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• pgi-2-- : pgi mutants utilize glucose by the hexose monoP shunt; strains with this allele grew on glucose at 1/3 parental rate. Slow growth on Gal,Mal,Lac;normal on Fru and gluconate.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant

References:

• Fraenkel, DG, SR Levisohn 1967. Glucose and gluconate metabolism in an Escherichia coli mutant lacking phosphoglucose isomerase. J. Bacteriol. 93(5):1571-8.
• Fraenkel, DG 1967. Genetic mapping of mutations affecting phosphoglucose isomerase and fructose diphosphatase in Escherichia coli. J. Bacteriol. 93(5):1582-7.