CGSC Strain#: 7732
Strain Designation: ALS217      Source of Strain: E. Altman
Sex: F- Episome/Plasmid: F128-xx
   Plasmid Markers/Mutations: lacZ4796::Tn5, lacIp-4003(I^Q1)
Chromosomal Markers: [araD139]_B/r, Δ(argF-lac)169, λ^-, e14-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsE0(SpcR), rbsR22, Δ(fimB-fimE)632(::IS1), deoC1
Strain Comments:

• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• flhD5301-- flagellar defect shown in Komeda and Iino
• Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
• Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
• ::IS1 = insertion of IS1
• fruA25 = former designation
• SpcR = spectinomycin resistant
• = kanamycin resistant

References:

• Warren, J., J. Walker, J.R. Roth, E. Altman 2000. Construction and characterization of a highly regulable expression vector pLAC11, and its multipurpose derivatives, pLAC22 and pLAC33. Plasmid 44:138-141