CGSC Strain#: 4907
Strain Designation: E. coli 15 TAU      Source of Strain: S.S. Cohen
Sex: F- Chromosomal Markers: car-92, thyA42, deoB20
Strain Comments:

• car-92-- E. coli 15 mutation.
• thyA42-- E. coli 15 mutation
• thyA42-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• deoB20-- E. coli 15 mutation.
• car-92 was formerly called pyrA38
• deoB20 was formerly called thyR32

References:

• BARNER, HD, SS COHEN 1958. Protein synthesis and RNA turnover in a pyrimidine-deficient bacterium. Biochim. Biophys. Acta 30(1):12-20.
• KANAZIR, D, HD BARNER, JG FLAKS, SS COHEN 1959. Some physiological and genetic properties of a strain of Escherichia coli requiring thymine, arginine and uracil. Biochim. Biophys. Acta 34:341-53.
• Stern, J.R. 1964. J.Mol.Biol. 8:629-0