CGSC Strain#: 7048
Strain Designation: ES1301      Source of Strain: E. Siegel
Sex: F- Chromosomal Markers: lacZ53(Am), λ^-, mutS201::Tn5, thyA36, IN(rrnD-rrnE)1, rha-5, metB1, deoC2, pur-
Strain Comments:

• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• pur--- : This strain requires adenine for growth on supplemented minimal medium. The Hfr grandparent carried purF1 but in a late-transferred region.
• = kanamycin resistant
• Am = amber(UAG) mutation

References:

• Siegel, EC, SL Wain, SF Meltzer, ML Binion, JL Steinberg 1982. Mutator mutations in Escherichia coli induced by the insertion of phage mu and the transposable resistance elements Tn5 and Tn10. Mutat. Res. 93(1):25-33.