CGSC Strain#: 8300
Strain Designation: BW20767      Source of Strain: B.L. Wanner
Other Designations: ATCC 47084
Sex: F- Chromosomal Markers: RP4-2(Km::Tn7,Tc::Mu-1), leu-163::IS10, ΔuidA3::pir⁺, recA1, endA1, thiE1, hsdR17, creC510
Strain Comments:

• Same as ATCC Strain No. 47084
• RP4-2(Km::Tn7,Tc::Mu-1)-- The RP4-2 also has an ISR1 insertion element within the Tn1³⁵ transposon that inactivates the adjacent ampicillin resistance gene.
• RP4-2(Km::Tn7,Tc::Mu-1)-- integrated plasmid is Kan and Tet sensitive, due to the insertions in Tc and Km, and the Tn7 results in TRIM/aminopterin resistance and low level strR.
• leu-163::IS10-- This allele was created by selecting for a spontaneous TetS version of leu-63::Tn10
• ΔuidA3::pir⁺-- Allows the replication of plasmids with an R6Kγ origin of replication
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• thiE1 was formerly called thi-1
• ΔuidA3::pir⁺ was formerly called uidA(Δ MluI)::pir⁺
• = good miniprep dsDNA quality
• Const = constitutive

References:

• Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.