CGSC Strain#: 5284
Strain Designation: HF4704      Source of Strain: T. Komano
Sex: F- Chromosomal Markers: thyA45, uvrA103, deo-225
Strain Comments:

• thyA45-- was called tlr-25 and thyR25, in conflict with the thyA25 allele. The Tlr (Thy low req.) phenotype is now known to result from two mutations, thyA^--, deo^-.
• thyA45-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• deo-225-- is in deoB or deoC. In combination with a thyA^- allele, it results in low Thy-requirement (Tlr) phenotype.
• deo-225 was formerly called thyA45
• deo-225 was formerly called thyR25
• deo-225 was formerly called tlr-25

References:

• Lindqvist, BH, RL Sinsheimer 1967. Process of infection with bacteriophage phi-X174. XIV. Studies on macromolecular synthesis during infection with a lysis-defective mutant. J. Mol. Biol. 28(1):87-94.
• Lindqvist, BH, RL Sinsheimer 1967. The process of infection with bacteriophage phi-X174. XV. Bacteriophage DNA synthesis in abortive infections with a set of conditional lethal mutants. J. Mol. Biol. 30(1):69-80.