CGSC Strain#: 5264
Strain Designation: Hfr 3000 U118      Source of Strain: F. Jacob
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacZ118(Oc), λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• lacZ118(Oc)-- is the lacZ allele originating in Paris strain Hfr 3000 U118. It is an ochre mutation.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• Oc = ochre(UAA) mutation

References:

• Newton, W.A., J.R. Beckwith, D. Zipser, S. Brenner 1965. Nonsense mutants and polarity in the lac operon of Escherichia coli. J.Mol.Biol. 14:290-296
• Zabin, I, AV Fowler, JR Beckwith 1978. Position of the mutation in beta-galactosidase ochre mutant U118. J. Bacteriol. 133(1):437-8.