CGSC Strain#: 5263
Strain Designation: Hfr 3000 X111      Source of Strain: F. Jacob
Other Designations: CA7033
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: Δ(gpt-lac)5, λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Beckwith, J.R., E.R. Signer 1966. Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi 80. J.Mol.Biol. 19:254-265