CGSC Strain#: 5257
Strain Designation: S3      Source of Strain: A. Garen
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, phoB63, ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant

References:

• Kreuzer, K.N., C. Pratt, A. Torriani 1975. Genetic analysis of regulatory mutants of alkaline phosphatase of Escherichia coli. Genetics 81:459-468
• Willsky, G.R., M. Malamy 1976. Control of the synthesis of alkaline phosphatase and the phosphate-binding protein in Escherichia coli. J.Bacteriol. 127:595-609