CGSC Strain#: 5243
Strain Designation: KA197      Source of Strain: W.P.M. Hoekstra
Other Designations: PC 0614
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, e14-, pheA97, relA1, spoT1, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Hoekstra, WP, PK Storm, EM Zuidweg 1974. Recombination in Escherichia coli. VI. Characterization of a recombination-deficient mutation with unusual properties. Mutat. Res. 23(3):319-26.