CGSC Strain#: 5230
Strain Designation: JC5029      Source of Strain: A.J. Clark
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: thr-300, λ^-, relA1, rpsE2300(SpcR), spoT1, ilv-318, thiE1
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• SpcR = spectinomycin resistant

References:

• Clark, A.J. 1967. The beginning of a genetic analysis of recombination proficiency. J.Cell.Physiol. 70:165-180
• Willetts, N.S., A.J. Clark, B. Low 1969. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. J.Bacteriol. 97:244-249