CGSC Strain#: 5227
Strain Designation: PL225, Rec-      Source of Strain: T. Fukasawa
Sex: F- Chromosomal Markers: Δ(nadA-galE)35, λ^-, recA1, relA1, rpsL104 or rpsL180(strR), spoT1, thiE1
Strain Comments:

• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• strR = streptomycin resist.

References:

• Mizuuchi, K, T Fukasawa 1969. Chromosome mobilization in rec-merodiploids of Escherichia coli K12 following infection with bacteriophage lambda. Virology 39(3):467-81.