CGSC Strain#: 7908
Strain Designation: TK2474/pAC-Gly(AS)      Source of Strain: W. Epstein
Sex: F- Episome/Plasmid: pAC-Gly(AS)
   Plasmid Markers/Mutations: glyU(AS*), cat(Am)
Chromosomal Markers: lacZ82(Am), nagA1, Δ(kdpC-kdpA)18, endA-, trkA405, kup-1, argE-(Am), thiE1
Strain Comments:

• 1. Please note this correction of a detail in t he plasmid description in the
• 2.reference below. The plasmid was cut with AvaI and EcoRV, rather than EcoRI and HindIII as stated on p. 9591, for insertion of the suppressor gene into pACYC184.
• lacZ82(Am)-- comes from strain Hfr 3000 X82. It is an amber mutation.
• Δ(kdpC-kdpA)18-- deletion has been called kdpABC5.
• argE-(Am)-- is suppressible by all of the characterized amber and ochre suppressors in Miller et a. 1977
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS* = synthetic amber suppressor mut

References:

• Dorus, S., H. Mimura, W. Epstein 2001. Substrate-binding clusters of the K+-transporting Kdp ATPase of Escherichia coli investigated by amber suppression. J.Biol.Chem. 276:9590-9598