CGSC Strain#: 5210
Strain Designation: ND25      Source of Strain: E. Ohtsubo
Sex: F' Episome/Plasmid: F100-11(F450)
   Plasmid Markers/Mutations: glnX44(AS)
   Plasmid Comment: Note discrepancy regarding {\it supE}$^{-}$ on F450.
Chromosomal Markers: Δ(galK-moaA)34, his-87, relA1, rpsL181(strR), spoT1
Strain Comments:

• (Note discrepancy regarding supE.)
• We have reports that F450 carries a supE mutation, probably supE44.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• strR = streptomycin resist.
• AS = amber(UAG) suppressor

References:

• Freifelder, D, A Folkmanis, I Kirschner 1971. Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles. J. Bacteriol. 105(3):722-7.
• Ohtsubo, E, MT Hsu 1978. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J. Bacteriol. 134(3):778-94.