CGSC Strain#: 7006
Strain Designation: RA2021      Source of Strain: C.R.H. Raetz
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, e14-, pssA21(ts), relA1, spoT1, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• ts = Temperature Sensitive

References:

• Raetz, CR, GD Kantor, M Nishijima, KF Newman 1979. Cardiolipin accumulation in the inner and outer membranes of Escherichia coli mutants defective in phosphatidylserine synthetase. J. Bacteriol. 139(2):544-51.