CGSC Strain#: 8211
Strain Designation: BW16829/pWM6      Source of Strain: B.L. Wanner
Sex: F' Episome/Plasmid: pWM6
   Plasmid Markers/Mutations: bla(ApR), uidA(p-), MCS-2, [aph](kan,neoR)
Episome/Plasmid: F128-Tn10-11
   Plasmid Markers/Mutations: lacIp-4000(lacI^Q), ΔlacZ58(M15), F::Tn10-11, proA , proB , Tn10-11 , lac
Chromosomal Markers: Δ(argF-lac)169, recA1, rpoS396(Am), rph-1, rob-1, creC510
Strain Comments:

• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Am = amber(UAG) mutation
• Const = constitutive
• = kanamycin resistant
• ApR = ampicillin resistant
• kan,neoR = from Tn903
• = tetracycline resistant
• M15 = alpha-complemented allele

References:

• Yanisch-Perron, C, J Vieira, J Messing 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33(1):103-19.
• Metcalf, W.W., B.L. Wanner 1993. Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement. Gene 129:17-25