CGSC Strain#: 7900
Strain Designation: XAC/pCys(AS)      Source of Strain: J.H.Miller/Promega
Sex: F- Episome/Plasmid: pCys(AS)
   Plasmid Markers/Mutations: bla(ApR), cysT(AS*)
Chromosomal Markers: Δ(gpt-lac)5, λ^-, ara(FG), gyrA-0(NalR), argE-(Am), rpoB0(rifR), thiE1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• argE-(Am)-- is suppressible by all of the characterized amber and ochre suppressors in Miller et a. 1977
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• NalR = Nalidix.acidResist.
• rifR = rifamycin resistance
• ApR = ampicillin resistant
• AS* = synthetic amber suppressor mut

References:

• Normanly, J, JM Masson, LG Kleina, J Abelson, JH Miller 1986. Construction of two Escherichia coli amber suppressor genes: tRNAPheCUA and tRNACysCUA. Proc. Natl. Acad. Sci. U.S.A. 83(17):6548-52.
• Normanly, J., L.G. Kleina, J.M. Masson, J. Abelson 1990. Construction of Escherichia coli amber suppressor tRNA genes. III. Determination of tRNA specificity. J.Mol.Biol. 213:719-726
• Anonymous 1994. Promega Technical Bull. -:189