CGSC Strain#: 8081
Strain Designation: CSH108      Source of Strain: J.H. Miller
Other Designations: XAC-1equivalent
Sex: F' Episome/Plasmid: F128-(CSH108)
   Plasmid Markers/Mutations: lacZ8(Am), lacI373
   Plasmid Comment: F-prime undesignated in Cold Spring Harbor manual. We assume it is an F128 derivative.
Chromosomal Markers: Δ(gpt-lac)5, λ^-, ara(FG), gyrA-0(NalR), argE-(Am), rpoB0(rifR), thiE1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• argE-(Am)-- is suppressible by all of the characterized amber and ochre suppressors in Miller et a. 1977
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• NalR = Nalidix.acidResist.
• rifR = rifamycin resistance
• Am = amber(UAG) mutation

References:

• Miller, J.H. 1992. Short Course in Bact.Genet. CSH Press 1:431