CGSC Strain#: 6826
Strain Designation: HG4      Source of Strain: H. Goldie
Sex: F- Chromosomal Markers: fhuA22, galK35, λ^-, pyrD34, pps-3, edd-1, his-68, tyrA2, relA1, rpsL125(strR), pck-2, malT1(λ^R), pitA10, xyl-7, mtlA2, spoT1, thiE1
Strain Comments:

• The succinate^- phentoype is tighter if cells are refrigerated (4hrs or more) before plating on minimal medium plus succinate.
• galK35-- is Brenner's gal^-_U99 from Hfr 3000 U99 and has been called galK99
• pps-3-- allele is called pps-1 by Fraenkel.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• LamR = Lambda-resistant
• strR = streptomycin resist.

References:

• Goldie, H., B.D. Sanwal 1980. Genetic and physiological characterization of Escherichia coli mutants deficient in phosphoenolpyruvate carboxykinase activity. J.Bacteriol. 141:1115-1121