CGSC Strain#: 5944
Strain Designation: PC5      Source of Strain: P. Carl
Sex: F- Chromosomal Markers: leuB6(Am), λ^-, thyA47, rpsL153(strR), dnaA5(ts), deoC3
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• thyA47-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• dnaA5(ts)-- is a temperature-sensitive mutation.
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Wechsler, JA, JD Gross 1971. Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol. Gen. Genet. 113(3):273-84.