CGSC Strain#: 6378
Strain Designation: Hfr 3000 YA694      Source of Strain: F. Jacob
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacI694(I^S), λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• lacI694(I^S)-- is a lac_i^S mutation. The repressor binds DNA, but not the inducer, so it cannot be induced and is Lac^-.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• I^S = super suppressor

References:

• Willson 1964. J.Mol.Biol. 8:582-0