CGSC Strain#: 6360
Strain Designation: KK446      Source of Strain: Fuchs & Karlstrom
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, lacY1, glnX44(AS), λ^-, nrdB2(ts), thyA6, rpsL67(strR), thiE1, deoC1, deoB37
Strain Comments:

• Although the mutation nrdB2 renders the strain temperature-sensitive, the mutant protein is not thermolabile.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Fuchs, J.A., H.O. Karlstrom 1976. Mapping of nrdA and nrdB in Escherichia coli K-12. J.Bacteriol. 128:810-814