CGSC Strain#: 7882
Strain Designation: BW27786      Source of Strain: B.L. Wanner
Sex: F- Chromosomal Markers: Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, Δ(araH-araF)570(::FRT), ΔaraEp-532::FRT, φP_cp13araE534, Δ(rhaD-rhaB)568, hsdR514
Strain Comments:

• Δ(araD-araB)567-- This deletion extends from ~25 bp upstream of the araB start codon to ~8 bp into the beginning of the araD gene
• ΔlacZ4787(::rrnB-3)-- : 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
• Δ(araD-araB)567 was formerly called Δ araBAD_AH33
• Δ(rhaD-rhaB)568 was formerly called Δ rhaBAD_LD78
• ΔlacZ4787(::rrnB-3) was formerly called ::rrnB-4
• ΔlacZ4787(::rrnB-3) was formerly called Δ lacZ_WJ16
• ::FRT = Flp Recombinase Target
• ::rrnB-3 = 4 copies of rrnB inserted

References:

• Khlebnikov, A., K.A. Datsenko, T. Skaug, B.L. Wanner, J.D. Keasling 2001. Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiol 147:3241-3247