CGSC Strain#: 8209
Strain Designation: BW18813/pBW30      Source of Strain: B.L. Wanner
Sex: F- Episome/Plasmid: pBW30
   Plasmid Markers/Mutations: oriR6K_γ, bla(ApR), oriT_RP4, bla , oriRg
Chromosomal Markers: Δ(codB-lacI)3, ΔphoA532, ΔuidA4::pir-116, rpoS396(Am), rph-1, Δ(phnP-phnD)3330( phnC?), [phn_B]
Strain Comments:

• pBW30 is the same as pLD54 except for PstI site in bla. pLD54 host is pir⁺, recA1, lacI^Q and CamR.
• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• ΔuidA4::pir-116-- Allows plasmids with an R6Kγ origin to replicate to high copy number
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• [phn_B]-- functional phn locus from E. coli B
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• Am = amber(UAG) mutation
• phnC? = may also delete phnC
• ApR = ampicillin resistant

References: