CGSC Strain#: 7870
Strain Designation: BW23474/pAH154      Source of Strain: B.L. Wanner
Sex: F- Episome/Plasmid: pAH154
   Plasmid Markers/Mutations: oriR6K_γ, tL3_λ(Ter), MCS, rgnB(Ter), P22(att), gen, MCS , tL3$\_{lambda}$ , attP22 , oriRg , gen-R , rgnB
Chromosomal Markers: Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am), endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510
Strain Comments:

• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• ΔuidA4::pir-116-- Allows plasmids with an R6Kγ origin to replicate to high copy number
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• endA9(del-ins)::FRT was formerly called endA_BT333
• Am = amber(UAG) mutation
• Const = constitutive
• del-ins = deletion-insertion
• Ter = terminator

References:

• Haldimann, A, BL Wanner 2001. Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. J. Bacteriol. 183(21):6384-93.