CGSC Strain#: 6212
Strain Designation: KL742      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: λ^-, thyA748::Tn10, rph-1, deo-77
Strain Comments:

• deo-77 is in deoB or deoC.
• thyA748::Tn10-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = tetracycline resistant

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