CGSC Strain#: 6186
Strain Designation: NK6051      Source of Strain: N. Kleckner
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: Δ(gpt-lac)5, purK79::Tn10, λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• purK79::Tn10 was formerly called purE79::Tn10
• thiE1 was formerly called thi-1
• = tetracycline resistant

References:

• Wanner, B.L. 1986. Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12. J.Mol.Biol. 191:39-58