CGSC Strain#: 5933
Strain Designation: NY73      Source of Strain: J.A. Wechsler
Sex: F- Chromosomal Markers: leuB6(Am), λ^-, thyA47, dnaG3(ts), IN(rrnD-rrnE)1, rpsL153(strR), metE70, polA1(Am), rpoB316, deoC3
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• thyA47-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Gefter, ML, Y Hirota, T Kornberg, JA Wechsler, C Barnoux 1971. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis. Proc. Natl. Acad. Sci. U.S.A. 68(12):3150-3.