CGSC Strain#: 6158
Strain Designation: Lin 225      Source of Strain: E.C.C. Lin
Sex: Hfr    Point of Origin#: 13   Map Position: 84.98   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: leuB6(Am), lacZ4, glnX44(AS), ptsI24, rpsL8, glpK31(fbR), thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• fbR = feedback resistant

References:

• Berman, M, EC Lin 1971. Glycerol-specific revertants of a phosphoenolpyruvate phosphotransferase mutant: suppression by the desensitization of glycerol kinase to feedback inhibition. J. Bacteriol. 105(1):113-20.
• Berman-Kurtz, M., E.C.C. Lin, D.P. Richey 1971. Promoter-like mutant with increased expression of the glycerol kinase operon of Escherichia coli. J.Bacteriol. 106:724-731