CGSC Strain#: 5853
Strain Designation: CS419      Source of Strain: C.R. Somerville
Sex: F- Chromosomal Markers: araC14, leuB6(Am), lacZ36, proC32, λ^-, hisF860(Stable), cysJ43, thyA54, rpsE2015(SpcR), rpsL109(strR), pyrE41, gpp-1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• cysJ43-- - the cys allele was formerly thought to be in cysC.
• thyA54-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• proC32 was formerly called proC65
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• SpcR = spectinomycin resistant
• Stable = stable, non-reverting
• strR = streptomycin resist.

References:

• Somerville, C.R., A. Ahmed 1979. Mutants of Escherichia coli defective in the degradation of guanosine 5'-triphosphate, 3'-diphosphate (pppGpp). Mol.Gen.Genet. 169:315-323